Copyright © 2020 Elsevier B.V. or its licensors or contributors. Studies on oriented and reversible immobilization of glycoprotein using novel boronate affinity gel.
Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. Using a 1:1 mixture of unlabeled acrylamide and deuterium-labeled acrylamide ([2,3,3'-D3]acrylamide), the proteins of interest were alkylated prior to electrophoretic separation. Copyright © 1992 Published by Elsevier Inc. https://doi.org/10.1016/0003-2697(92)90013-W. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Cysteine alkylation in unprotected peptides: synthesis of a carbavasopressin analog by intramolecular cysteine alkylation. This is Publication 111 from the Arizona State University Center for the Study of Early Events in Photosynthesis. Your Mendeley pairing has expired. Alkylation of cysteine in proteins with acrylamide under mildly alkaline conditions yields a thioether derivative, Cys-S-β-propionamide (Cys-S-Pam), which is stable during automated Edman degradation.Its phenylthiohydantoin derivative, PTH-Cys-S-Pam, is easily separated from other PTH-amino acids by HPLC and is thus useful for cysteine identification during protein sequencing. 8 publications. Yan Feng, Donald R. Menick, Bernice M. Katz, Charles J. Beischel, E. Starr Hazard, Saurav Misra, Thomas G. Ebrey, and Rosalie K. Crouch. Get article recommendations from ACS based on references in your Mendeley library. These metrics are regularly updated to reflect usage leading up to the last few days. An improved method for cysteine alkylation. Using a 1:1 mixture of unlabeled acrylamide and deuterium-labeled acrylamide ([2,3,3‘-D 3 ]acrylamide), the proteins of interest were alkylated prior to electrophoretic separation. Synthetic α-helical peptides incorporating intercalators for DNA recognition. Find more information on the Altmetric Attention Score and how the score is calculated.
Alkylation with iodoacetamide after cystine reduction results in the covalent addition of a carbamidomethyl group (57.07 Da) and prevents the formation of … Gemma Triola, Luc Brunsveld and Herbert Waldmann. Alkylation of cysteine-containing peptides J. Peptide Res. Methods are also presented for alkylation of cysteine in proteins adsorbed on fiberglass disks in the reaction cartridge of a protein sequencer. Alkylation of cysteine in proteins with acrylamide under mildly alkaline conditions yields a thioether derivative, Cys-S-β-propionamide (Cys-S-Pam), which is stable during automated Edman degradation. ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. Alkylation of cysteine with acrylamide for protein sequence analysis.
Racemization-Free Synthesis of S-Alkylated Cysteines via Thiol-ene Reaction. Alkylation with NEM is a measure of the reactivity and/or accessibility of a given Cys residue to this small, relatively hydrophobic, membrane-permeant thiol-specific reagent. You’ve supercharged your research process with ACS and Mendeley! Iodoacetamide is a sulfhydryl-reactive alkylating reagent used to block reduced cysteine residues for protein characterization and peptide mapping. 55, 2000 / 140–147 | 141. from those of the unreacted starting reagents, and thus the new compound was a candidate for 1.